Supplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2d nmr








télécharger 64.7 Kb.
titreSupplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2d nmr
date de publication18.11.2017
taille64.7 Kb.
typeDocumentos
Supplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2D NMR
Tangi Jézéquel1,2, Catherine Deborde2,3, Mickaël Maucourt2,4, Vanessa Zhendre2, Annick Moing2,3 and Patrick Giraudeau1,5




1 EBSI Team, Chimie et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes, CNRS, UMR 6230, LUNAM Université, F-44322 Nantes, France

2 Plateforme Métabolome Bordeaux-MetaboHUB, Centre de Génomique Fonctionnelle Bordeaux, IBVM, Centre INRA Bordeaux, 71 av Edouard Bourlaux, 33140 Villenave d’Ornon, France

3 INRA, UMR1332, Biologie du Fruit et Pathologie, Centre INRA Bordeaux, 71 av Edouard Bourlaux, 33140 Villenave d’Ornon, France

4 Université de Bordeaux, UMR 1332 de Biologie du Fruit et Pathologie, Centre INRA Bordeaux, F-33140 Villenave d’Ornon, France

5 Institut Universitaire de France, 1 rue Descartes, 75005 Paris, France

*Corresponding author:

Dr. Patrick GIRAUDEAU, Chimie et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), UMR 6230, Faculté des Sciences, BP 92208, 2 rue de la Houssinière, F-44322 Nantes Cedex 03, France. Tel. +33(0)2 51 12 57 09. Fax. +33(0)2 51 12 57 12.

E-mail: patrick.giraudeau@univ-nantes.fr
Supplementary Table S1. Biological characteristics of the tomato fruit pericarp extracts used in this study. For each sample, extracts A and B were systematically pooled before the NMR analysis. Other experimental details are given in the text.


Extract

Mass

(mg)

Biological evolution (DPA)

Truss




Extract

Mass

(mg)

Biological evolution (DPA)

Truss

10 A

20,0

8

5




490 A

20,2

34

5

10 B

19,8

8

5




490 B

19,8

34

5

118 A

19,9

8

6




514 A

20,2

34

6

118 B

19,9

8

6




514 B

20,3

34

6

406 A

19,9

8

7




1086 A

20,3

34

7

406 B

20,0

8

7




1086 B

20,0

34

7

394 A

19,7

21

5




1028 A

20,2

55

5

394 B

20,3

21

5




1028 B

19,9

55

5

418 A

20,0

21

6




1074 A

20,5

55

6

418 B

20,2

21

6




1074 B

20,2

55

6

478 A

19,6

21

7




1170 A

20,0

55

7

478 B

19,9

21

7




1170 B

20,0

55

7


DPA: Days Post Anthesis

Supplementary Table S2. Concentration (mmol.L-1) of the calibration samples containing the 8 targeted metabolites.


Sample

[Glc]

[Fru]

[Gln]

[Cit]

[Suc]

[GABA]

[Mal]

[Cho]



MW

180.16

180.16

146.14

192.12

342.30

139.12

134.09

104.17




1

100

10

10

15

10

0.3

3

0.2

148

2

75

25

7.5

12

7

0.6

6

0.5

125

3

50

50

5

9

5

0.9

9

0.5

128

4

25

75

2.5

6

3

1.2

12

0.65

121

5

10

100

0.5

3

0.5

1.5

15

0.8

128


Glc: Glucose; Fru: Fructose; Gln: Glutamine; Cit: Citric Acid; Suc: Sucrose; GABA: Gamma-aminobutyric acid; Mal: Malic acid; Cho: Choline. MW: Molecular weight in g.mol-1.
Supplementary Table S3. UF COSY NMR peaks used for the quantification of the 8 targeted metabolites.

ChEBI

Name

Abbreviation

UF COSY peak used for quantification

500 MHz

700 MHz

F2 (ppm)

F1 (ppm)

F2 (ppm)

F1 (ppm)

17234

Glucose

Glc

3.31

0.74F

3.25

4.61

28757

Fructose

Fru

4.12

4.12

4.12

4.12

28300

Glutamine

Gln

2.47

2.15

1.91

3.01

30769

Citric acid

Cit

2.57

2.69

2.57

2.69

17992

Sucrose

Suc

4.22

4.22

4.22

4.22

15887

GABA

GABA

-

-

3.01

1.91

6650

Malic acid

Mal

2.67

2.38

2.67

2.38

15354

Choline

Cho

-

-

3.20

3.20

F: Folded peak along the F1 dimension. The true chemical shift is 4.61 ppm

similaire:

Supplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2d nmr iconProton nmr quantitative profiling for quality assessment of greenhouse-grown tomato fruit

Supplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2d nmr iconProton nmr quantitative profiling for quality assessment of greenhouse-grown tomato fruit

Supplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2d nmr iconSupplementary material

Supplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2d nmr iconSupplementary (Supporting) material

Supplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2d nmr iconElectronic Supplementary Material 1 Beisel et al

Supplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2d nmr iconHost plant range of a fruit fly community (Diptera: Tephritidae):...
«Centre de Biologie pour la Gestion des Populations», inra-spe, 755 avenue du Campus, Agropolis, cs 30016, 34988 Montferrier sur...

Supplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2d nmr iconHost plant range of a fruit fly community (Diptera: Tephritidae):...
«Centre de Biologie pour la Gestion des Populations», inra-spe, 755 avenue du Campus, Agropolis, cs 30016, 34988 Montferrier sur...

Supplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2d nmr iconLe programme de «Fast-Track»

Supplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2d nmr iconIntra individual variability in fecal cortisol metabolites varies...

Supplementary material for: Absolute quantification of metabolites in tomato fruit extracts by fast 2d nmr iconIntra individual variability in fecal cortisol metabolites varies...








Tous droits réservés. Copyright © 2016
contacts
b.21-bal.com