Supplementary Information to Amphipols and photosynthetic








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titreSupplementary Information to Amphipols and photosynthetic
date de publication17.05.2017
taille12.71 Kb.
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Supplementary Information to

Amphipols and photosynthetic

light-harvesting pigment-protein complexes



Milena Opačić1, Grégory Durand2,3,

Michael Bosco2,3, Ange Polidori2,3, Jean-Luc Popot4

1VU University Amsterdam- Faculty of Sciences, Department of Physics and Astronomy, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands;
2Université d'Avignon, Equipe Chimie Bioorganique et Systèmes Amphiphiles, 33 rue Louis Pasteur, F84000 Avignon, France;
3Institut des Biomolécules Max Mousseron (UMR 5247), 15 avenue Charles Flahault, F-34093 Montpellier Cedex 05, France.
4Unité Mixte de Recherche 7099, Centre National de la Recherche Scientifique and Université Paris 7, Institut de Biologie Physico-Chimique, 13 rue Pierre-et-Marie Curie, F-75005 Paris, France;



Scheme 1 Synthesis of NAPol NA13. For details, see Sharma et al. 2012.
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Fig. S1 Size exclusion chromatography profiles of A8-35-trapped Lcb1 (grey) and of Lhcb1 in αDDM (black), normalized to the same maximum. Detection wavelength: 439 nm.

description: description: lhcii emission spectra 170113.tif

Fig. S2 Fluorescence emission spectra of A8-35- and NA13-trapped LHCII and of LHCII kept in 0.03% αDDM. Excitation wavelength is 475 nm.




Fig. S3 Sucrose gradients analysis of LHCII trimers. A) NA13-trapped, pH 5.5; B) in 0.03% αDDM, pH 5.5; C) NA13-trapped, pH 6.5; D) in 0.03% αDDM, pH 6.5; E) NA13-trapped, pH 7.6; F) in 0.03% αDDM, pH 7.6.

Reference
Sharma, KS, Durand, G, Gabel, F, Bazzacco, P, Le Bon, C, Billon-Denis, E, Catoire, LJ, Popot, J-L, Ebel, C, Pucci, B (2012). Non-ionic amphiphilic homopolymers: Synthesis, solution properties, and biochemical validation. Langmuir 28:4625-4639


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